International Journal of Hematology and Oncology 2019, Vol 29, Num 3 Page(s): 157-167
Lamin Protein Gene Expression in Childhood Acute Lymphoblastic Leukemia

Ayse OZKAN1, Ibrahim BAYRAM1, Gulay SEZGIN1, Can ACIPAYAM1, Serhan KUPELI1, Atila TANYELI1

Cukurova University Faculty of Medicine, Department of Pediatric Oncology and Pediatric Bone Marrow Transplantation Unit, Adana,TURKEY

Keywords: Acute lymphoblastic leukemia, Childhood, Lamin proteins
Acute lymphoblastic leukemia (ALL) is the most common type of cancer in children. The identification of prognostic factors has become essential in design and analysis of modern therapeutic trials. The aim of this study is to present the expression patterns of lamin A/C, lamin B1 and B2 and their prognostic value in childhood acute lymphoblastic leukemias, which are thought to be related with cell proliferation and apoptosis. The study was carried out between January 2008 and March 2014 at the Cukurova University Faculty of Medicine, Division of Pediatric Oncology and sixty-four patients diagnosed with ALL and 35 control cases were included in the study. Real Time-Polymerase Chain Reaction (RT-PCR) method was used to detect lamin A/C, lamin B1 and lamin B2 protein expression at the time of the diagnosis and at the end of the induction treatment. At the time of the diagnosis, lamin B1 protein gene expression was lower in ALL patients when compared to the control group (p= 0.001). When lamin gene expression levels at the time of diagnosis and after induction therapy were compared, the lamin A/C and B2 gene expressions were significantly lower after the administration of induction chemotherapy (both values p= 0.0001). The decrease in lamin B1 gene expression was not statistically significant (p= 0.403). When the lamin expression levels in the control group were compared with the levels after induction chemotheraphy, lamin expressions were found to be significantly lower (all p values 0.0001). In childhood ALL, lamin B1 protein gene expression could be used as a diagnostic marker, while decrease in the lamin A/C and B2 protein gene expressions after induction could be used as a marker for response to therapy. Our study is the first to show in lamin protein gene expression relationship with diagnosis and prognosis in ALL in literature. Comprehensive multicenter studies are needed with a larger cohort of patients to reach more reliable results.